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trpm8 antagonist experiment  (MedChemExpress)
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MedChemExpress trpm8 antagonist experiment
( A – C ) Gene expression of TRPV1 ( A ), TRPV4 ( B ) and <t>TRPM8</t> ( C ) in the hindpaw skin in ST2−/− mice at 4 h after IL-33 injection (n = 5-7 mice). D Colocalization of TRPV1(upper panel) and TRPM8(lower panel) with PGP9.5 in the hindpaw (n = 3 mice, scale bar: 100 μm). Blocking TRPV1 with AMG9810 attenuated IL-33-induced mechanical ( E ) and thermal hypersensitivity ( F ) (n = 6 mice). G Blocking TRPV1 with capsazepine (CPZ) attenuated IL-33-induced thermal hypersensitivity (n = 6 mice). H Blocking TRPM8 with AMG2850 attenuated IL-33-induced cold allodynia (n = 6). I Expression of TRPV1 in DRGs (n = 3 mice, scale bar: 100 μm). J Quantification of ( I ), TRPV1-postive number in DRGs(n = 3 mice). K Colocalization of CCR2 with PGP9.5 in the hindpaw (n = 3 mice, scale bar: 100 μm). Intraplantar injection of CCL2 induces mechanical ( L ) and thermal hypersensitivity ( M ) (n = 6 mice). N Representative LTP traces show c-fiber-evoked field potentials in the dorsal horn of spinal cord in various inflammatory pain models. O Quantification of ( N ) (n = 5 rats). P Representative LTP traces show c-fiber-evoked field potentials in the dorsal horn of spinal cord by pretreatment with AMG9810 and AMG2850 in CCL2-induced model (n = 3 rats). Data are presented as mean ± SEM. * p < 0.01, ** p < 0.01, **** p < 0.0001, # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001, $ p < 0.05, $$ p < 0.01, $$$$ p < 0.0001. # g represents WT vs ST2−/− or vehicle vs drug treatment and $ represents PBS+veh vs IL-33+drug treatment (0.5 μg CPZ in ( G ) or AMG2850 in ( H )). One-way ANOVA with Bonferroni’s post-hoc test for ( A – C, E , F ), unpaired t-test for ( J , O , P ), Repeated measures for two-way ANOVA with Bonferroni’s post-hoc test for ( G , H, L , M ).
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( A – C ) Gene expression of TRPV1 ( A ), TRPV4 ( B ) and TRPM8 ( C ) in the hindpaw skin in ST2−/− mice at 4 h after IL-33 injection (n = 5-7 mice). D Colocalization of TRPV1(upper panel) and TRPM8(lower panel) with PGP9.5 in the hindpaw (n = 3 mice, scale bar: 100 μm). Blocking TRPV1 with AMG9810 attenuated IL-33-induced mechanical ( E ) and thermal hypersensitivity ( F ) (n = 6 mice). G Blocking TRPV1 with capsazepine (CPZ) attenuated IL-33-induced thermal hypersensitivity (n = 6 mice). H Blocking TRPM8 with AMG2850 attenuated IL-33-induced cold allodynia (n = 6). I Expression of TRPV1 in DRGs (n = 3 mice, scale bar: 100 μm). J Quantification of ( I ), TRPV1-postive number in DRGs(n = 3 mice). K Colocalization of CCR2 with PGP9.5 in the hindpaw (n = 3 mice, scale bar: 100 μm). Intraplantar injection of CCL2 induces mechanical ( L ) and thermal hypersensitivity ( M ) (n = 6 mice). N Representative LTP traces show c-fiber-evoked field potentials in the dorsal horn of spinal cord in various inflammatory pain models. O Quantification of ( N ) (n = 5 rats). P Representative LTP traces show c-fiber-evoked field potentials in the dorsal horn of spinal cord by pretreatment with AMG9810 and AMG2850 in CCL2-induced model (n = 3 rats). Data are presented as mean ± SEM. * p < 0.01, ** p < 0.01, **** p < 0.0001, # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001, $ p < 0.05, $$ p < 0.01, $$$$ p < 0.0001. # g represents WT vs ST2−/− or vehicle vs drug treatment and $ represents PBS+veh vs IL-33+drug treatment (0.5 μg CPZ in ( G ) or AMG2850 in ( H )). One-way ANOVA with Bonferroni’s post-hoc test for ( A – C, E , F ), unpaired t-test for ( J , O , P ), Repeated measures for two-way ANOVA with Bonferroni’s post-hoc test for ( G , H, L , M ).

Journal: Communications Biology

Article Title: IL-33/ST2 drives inflammatory pain via CCL2 signaling and activation of TRPV1 and TRPM8

doi: 10.1038/s42003-025-08119-3

Figure Lengend Snippet: ( A – C ) Gene expression of TRPV1 ( A ), TRPV4 ( B ) and TRPM8 ( C ) in the hindpaw skin in ST2−/− mice at 4 h after IL-33 injection (n = 5-7 mice). D Colocalization of TRPV1(upper panel) and TRPM8(lower panel) with PGP9.5 in the hindpaw (n = 3 mice, scale bar: 100 μm). Blocking TRPV1 with AMG9810 attenuated IL-33-induced mechanical ( E ) and thermal hypersensitivity ( F ) (n = 6 mice). G Blocking TRPV1 with capsazepine (CPZ) attenuated IL-33-induced thermal hypersensitivity (n = 6 mice). H Blocking TRPM8 with AMG2850 attenuated IL-33-induced cold allodynia (n = 6). I Expression of TRPV1 in DRGs (n = 3 mice, scale bar: 100 μm). J Quantification of ( I ), TRPV1-postive number in DRGs(n = 3 mice). K Colocalization of CCR2 with PGP9.5 in the hindpaw (n = 3 mice, scale bar: 100 μm). Intraplantar injection of CCL2 induces mechanical ( L ) and thermal hypersensitivity ( M ) (n = 6 mice). N Representative LTP traces show c-fiber-evoked field potentials in the dorsal horn of spinal cord in various inflammatory pain models. O Quantification of ( N ) (n = 5 rats). P Representative LTP traces show c-fiber-evoked field potentials in the dorsal horn of spinal cord by pretreatment with AMG9810 and AMG2850 in CCL2-induced model (n = 3 rats). Data are presented as mean ± SEM. * p < 0.01, ** p < 0.01, **** p < 0.0001, # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001, $ p < 0.05, $$ p < 0.01, $$$$ p < 0.0001. # g represents WT vs ST2−/− or vehicle vs drug treatment and $ represents PBS+veh vs IL-33+drug treatment (0.5 μg CPZ in ( G ) or AMG2850 in ( H )). One-way ANOVA with Bonferroni’s post-hoc test for ( A – C, E , F ), unpaired t-test for ( J , O , P ), Repeated measures for two-way ANOVA with Bonferroni’s post-hoc test for ( G , H, L , M ).

Article Snippet: For TRPM8 antagonist experiment, AMG2850 (1 μg/10 μL, MCE, HY-104059) or 10 μL vehicle was intraplantarly delivered 30 min before IL-33 injection (3626-ML/CF, 200 ng/20 μL).

Techniques: Gene Expression, Injection, Blocking Assay, Expressing